Mitotic Spindle

This volume includes a series of§protocols focused on mitotic spindle assembly and function. The methods covered§in this book feature a broad range of techniques from basic microscopy to the§study of spindle physiologies relevant to cancer. These methods can be applied§to diverse model systems that range from the cell-free Xenopus egg§extract system to the moss Physcomitrella patens , in an effort to§demonstrate the key contributions made by researchers using multiple model§organisms. Chapters in The Mitotic Spindle: Methods and Protocols integrate cutting-edge technologies that have only become available due to the§cross-disciplinary efforts, such as ATP analogue sensitive inhibition of§mitotic kinases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics,§lists of the necessary materials and reagents, step-by-step, readily§reproducible laboratory protocols, and tips on troubleshooting and avoiding§known pitfalls.§§Thorough and informative, The§Mitotic Spindle: Methods and Protocols, is a valuable resource for§researchers who are new to mitosis or are already experts in the field.§§