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Single Molecule Enzymology

Name: Single Molecule Enzymology
Brand: Humana Press Inc.
SKU: 01432609
Price: 1040.28 RON
Availability: InStock
Author: Gregory I. Mashanov
ISBN: 9781617792601

Methods and Protocols

Gregory I. Mashanov

Christopher Batters

Limba

engleză

Carte Copertă tare

Codul Libristo: 01432609

Editura Humana Press Inc., august 2011

The last fifteen years have witnessed the birth and maturation of many original methods and the deve... Descrierea completă

Codul Libristo: 01432609

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The last fifteen years have witnessed the birth and maturation of many original methods and the development of protocols specific to single molecule measurements and their analysis, including techniques involving optical imaging, electron microscopy, optical and magnetic trapping, and developments in atomic force microscopy. In Single Molecule Enzymology: Methods and Protocols, experts in the field provide procedures which enable the extraction of detailed information about enzyme work cycles, their static and kinetic properties, and information about their location and activity within cells. The detailed volume offers practical advice on many aspects of single molecule enzymology and includes strategic overviews of interconnected methods involved in sample preparation, single molecule measurements, and data analysis. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.§Authoritative and up-to-date, Single Molecule Enzymology: Methods and Protocols is intended for use within the diverse community of molecular biologists, biochemists, and biophysicists studying enzymes in detail and can be used by researchers planning their first single molecule study or to aid more experienced researchers in further developing their existing studies.