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R3: Relative Rapid Resolution
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This is encouraging from both practical and scientific view points; efficacy has often been proven over the centuries. Improvements in instrumentation, robotics and bioassay technology have increased the speed, resolution and sensitivity. The use of a commercially available elevated pressure HPLC system has been shown to be beneficial in the pharmaceutical development area.UPLC using 1.7µm particles and a properly holistically designed system provide significantly more resolution while reducing run time, and improve sensitivity for the analyses of many compounds. At a time when many scientists have reached separation barriers with conventional HPLC, UPLC presents the possibility to extend and expand the utility of chromatography. New UPLC technology in both chemistry and instrumentation boosts productivity by providing more information per unit of work as UPLC fulfils the promise of increased resolution, speed, and sensitivity predicted for liquid chromatography.
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