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The book is about the protein stability and conformational changes in different ionic liquid environment. It represents the biocompatibility of the imidazolium-based ionic liquids (ILs), which has been investigated by studying their impact on the structure and stability of Bovine serum albumin (BSA) and Lysozyme (Lyz) using different spectroscopic techniques. As well as, elucidation of the mechanism of interaction between the proteins and ILs is provided.