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Nitric oxide, recently named "Molecule of the Year" by the journal "Science", impinges on a wide range of fields in biological research, particularly in the areas of biomedicine and cell and organismal biology, as well as in fundamental chemistry. This volume and its companion Volume 269 highlight the most current and up-to-date methods used to research the physiology and toxicity of nitric oxide in cellular and organismal systems. It will be a valuable resource for the experienced researcher as well as for those newly entering the field. Key features include: generation, detection, and characterization of biological and chemical sources of nitric oxide; and, biochemistry and molecular biology of enzymes and proteins associated with nitric oxide metabolism.