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Molecular Beacons explains working principle of molecular beacons, discusses the design, synthesis, purification and characterization of molecular beacons, explores their thermodynamic and kinetic properties of molecular beacons, and more importantly, reviews their in vivo and in vitro applications with the emphasis on the design and modification of molecular beacons for in vivo mRNA imaging applications.§Molecular Beacons is designed to bring together in a single resource an organized and comprehensive view of molecular beacons and will be a valuable resource for academic, clinical and industrial scientists and graduate students who may consider exploring molecular beacons in their research or practice.§Weihong Tan is the University of Florida Distinguished Professor and V. T. and Louis Jackson Professor of Chemistry at the University of Florida, USA.§Chaoyong James Yang is the Lu Jiaxi Professor of Chemistry at Xiamen University, China.