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This book will provide a detailed history and discussion of the use of human tissues as an alternative to animal-based testing for assessing the efficacy and safety of new medicines. Beginning by providing the history and background of animal-based testing, this text then describes in detail the issues relating to access to human cells and tissue and the rules and regulations governing their use. The book contains chapters on tissue slices, culture, assays, microfluidics and body-on-a-chip technology, as well as chapters on emerging technologies and techniques such as engineered organs. Each chapter comprehensively covers a technique, from its utilisation and methodology to the opportunities and limitations of the method. Written and edited by Worldwide experts within the field, this book will be an essential text for biochemists and researchers working in translational medicine in both industry and academia.