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Candida albicans biofilm formation is a poorly§understood, complex biological process with§significant consequences for human health. In this§thesis, I describe the progress I have made in§elucidating the genetic mechanisms of Candida§albicans biofilm formation using a large-scale gene§disruption strategy. The Bcr1-dependent adherence§regulatory pathway that I discovered is pivotal to§our understanding of C. albicans biofilm formation. §Without the transcription factor Bcr1, named for§Biofilm and Cell-wall Regulator, C. albicans cells§are unable to form biofilms, a process that is§extremely important to its lifestyle and§pathogenicity. Expression analysis indicates that§Bcr1 activates cell surface protein and adhesin§genes, whose gene products could be used to develop§new anti-fungal therapies.