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Presenting§state-of-the-art protocols to study gap junctions, this detailed book first§focuses on the use of methods and tools to investigate the different aspects of§connexin expression and gap junction regulation. The second part of the volume§describes several methods to probe gap junction functionality as such. Written§in the highly successful Methods in Molecular Biology series format, chapters§include introductions to their respective topics, lists of the necessary§materials and reagents, step-by-step and readily reproducible laboratory§protocols, and key tips on troubleshooting and avoiding known pitfalls. § Authoritative§and practical, Gap Junction Protocols is intended for basic and applied§researchers in the area of biomedical and life sciences, both in academic and§industrial settings.