Essentials in Modern HPLC Separations

Essentials in Modern HPLC Separations, Second Edition discusses the role of separation in high performance liquid chromatography (HPLC). Chromatographic analysis and the high-performance liquid chromatography (HPLC) are the most utilized analytical method for obtaining information about the chemical composition of a variety of materials and processes. This new and updated edition systematically presents the basic concepts as well as the new developments in HPLC. Starting with a description of basic concepts, it provides important guidance for practical utilization of various HPLC procedures, such as selection of the HPLC type, proper choice of the chromatographic column, selection of mobile phase, selection of the method of detection, in correlation with the physico-chemical characteristics of the compounds to be separated and sample matrix. Essentials in Modern HPLC Separations, Second Edition includes the newest trends and contributions to HPLC separation, from both theoretical and applicative points of view. Every chapter has been carefully reviewed and several new sections have been added to bring the book completely up to date. Hence, it is a valuable reference for students and professors in chemistry. Thoroughly updated throughout, with an entirely new section on Computer-aided Method Development in HPLC and new subsections on miniaturization and automation in HPLC, chemometric aspects of HPLC, green solvent use in HPLC, and more Provides insights into the chromatographic process to find the optimum solution for analyzing complex samples Provides a basis for understanding the utilization of modern HPLC for applications, particularly for the analysis of pharmaceutical, biological, food, and beverage, and environmental samples