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Ectomycorrhizal fungi play multifunctional role during symbiosis with higher plants. They can serve as bioprotectors, biofertilizers, bioremediators and stress indicators and they are the true mycoindicators of the forest ecosystem. In a forest an enormous diversity of ectomycorrhizal fungi can be found. Moreover, some ectomycorrhizal fungi produce edible sporocarps, fruiting bodies, which are important for the food industry.§Ectomycorrhizal fungi produce various metal chelating molecules, which have remarkable biotechnological significance and also secrete useful secondary metabolites. Molecular approaches are required for the identification and differentiation of the fungi forming symbiosis with higher plants and molecular tools are important to understand how genes are expressed during symbiosis with higher plants.