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The main goal of the radiation therapy is to kill the cancerous cell and save the normal cells with treatment planning. The sophisticated Treatment Planning Systems (TPSs) are used to improve the accuracy of dose calculations in radiotherapy treatment planning. Regarding patient safety and success of therapy, its accurate and stable functioning is an issue of highest importance. For these reasons commissioning and Quality Assurance (QA) procedures have to be implemented for the clinical routine running of a TPS. The purpose of this work is to perform the commissioning and Quality Assurance (QA) of Eclipse Treatment Planning System (TPS).