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Using contextualizing theories of wildlife and wildlife crime internationally and in South Africa, this brief focuses on the practical use of gelatin lifters, their analysis and evaluation. It evaluates the use, suitability and performance of gelatin lifters for the visualization and sequential processing of finger-, shoe- and footprints, and environmental traces. Using South African contexts, it makes recommendations for practice based on the growing cooperation of international forces, regional and local actors in combating wildlife crime. As global and local programs, actions and law enforcement strategies to combat wildlife crime are gaining strength, forensic trace evidence provides one useful method of law enforcement in combating wildlife crime. This brief is suitable for students and researchers in forensic science, wildlife crime, green criminology, as well as law enforcement organizations combating wildlife crime.