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The reaction rate for OH + CH3COOH is first measured at 7 Torr and found to have a strong negative temperature dependence. An Arrhenius expression of k(T) = (5.38 ± 0.28) x 10~14exp (740 ± 51 / T) cm3molecule"]s'1is obtained.The reaction between the hydroxyl radical (OH) and acetic acid (CH3COOH) is important in upper troposphere/lower stratosphere, its role comparable to the reaction of OH with methane in controlling the tropospheric oxidative capacity.