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This volume focuses on the transient gene expression technologies for recombinant protein production and gene therapy. It provides coverage of an important issue in Cell Engineering by internationally authoritative experts. Topics covered range from the use of baculovirus, adenovirus, adeno-associated virus and alphavirus vectors in biopharmaceutical, genomics and gene delivery research, to large-scale transfection of plasmid DNA into cultured cells for bioreactor applications. This book illuminates all the scientific and technological issues involved in the research, development, production, and human use of viral and non-viral vectors for in vitro and in vivo expression of recombinant proteins, thus making it an essential reference for cell culture technologists, virologists, and scientists interested in gene therapy.