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Particles on the nano-scale exhibit extraordinary properties and offer exciting opportunities in a wide range of applications, including visualization of cellular structures, receptors, inflammation, and characterisation of suspect lesions. The aim of this Symposium was to gather scientists active in the field (chemists, material scientists, physicists, engineers, clinicians and biochemists) to discuss new ideas, experimental results and perspectives ranging from the preparation to the applications of functional nanoparticles for bio-imaging and cell labelling.