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The book covers all the fundamental principles for researchers both fresh and professionals who are targeting the field of Biomaterials and Tissue Engineering. Biomaterials and Tissue Engineering is an interdisciplinary field of research with perspectives and contributions from scientists, engineers, and physicians. With a strong focus on biomaterials and scaffolds, the book also covers the testing and evaluation pathway for in vitro and in vivo studies. This book presents a broad range of topics related to the fundamentals of 3D printing and bioprinting, and the collection goes on to describe contemporary technology used in tissue engineering applications as well as currently available biomaterials suitable for tissue repair and regeneration. Fundamentals of tissue engineering and the ideal conditions to fabricate scaffolds and to prepare biomaterials are discussed, followed by the elucidation of individual materials used in tissue engineering. This book provides to present the current 3D printing techniques and scaffold production techniques for tissue engineering applications from the perspectives of professionals from different disciplines. Therefore, the main purpose of the book is to provide: (i) why humans need biomaterials, especially in medical applications, (ii) different types of tissue engineering strategies, and (iii) modeling, characterization, and evaluation of outputs of those strategies. The authors strongly believe that the proposed book is a reference material in the field of tissue engineering, where bachelor, M.Sc., and Ph.D. students, researchers, academicians, medical industry, and healthcare professionals from diverse backgrounds are involved.