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Considering that the beagle dog is routinely used in toxicology as a non rodent species and more broadly in the field of neuroscience research, the idea of building a new reference in the field arose, in the hope that an up-to-date and readily available atlas decreases the need to use dogs as controls or for teaching purposes. Moreover, the work serves as a guide to understand the comparative brain structure of other mammals, because although some important differences do exist, particularly in the nomenclature of the cortical sulci and gyri, the similarity of the arrangement of nuclei and tracts is striking.