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Activity-Based Protein Profiling
Design and synthesis of activity-based probes (ABPs), adaptation of activity-based protein profiling (ABPP) for plant proteomes studies
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Until now, the only technology to directly demonstrate the role of switching between the active/inactive states of enzymes during plant defence is ABPP. Since the discovery of ABPP ten years ago, several ABPs have been developed and used in pharmacological and physiological studies. However, a broader use of ABPP is still limited by the availability of suitable probes. In an attempt to expand this limited applicability of ABPP, in this thesis, new types of ABPs were designed, synthesized and evaluated as potential probes for the study of plant-pathogen interactions. To develop effective ABPs in an efficient way, different design strategies have been tested and discussed accompanying with five questions, to generate different types of novel ABPs ...
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