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A Novel Method for ICSI: Rotationally Oscillating Drill
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ICSI (intracytoplasmic sperm injection) procedure is§one of the most commonly used cellular-injection§processes. In most advanced applications a piezo§actuator creates impulsive forcing. This procedure§presently requires a very small mercury column inside§the glass pipette which is helpful especially for§minimizing the transverse oscillations. A novel§microinjection procedure, which will remedy the§shortfalls of the present technology is introduced.§The highlight of this procedure is the introduction§of rotational oscillations to the pipette during the§drilling. These oscillations of small amplitudes and§high enough frequencies are shown to create very§effective piercing. The so-called Ros-Drill© is a§mercury-free and minimally invasive device which is§designed and built including the peripheral control§hardware and software.
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